Pipeline Optimization Parameter

2D Kernel Convolution

2D DFT Filter

2D DFT Filter Butterworth BP

2D DFT Filter Butterworth BP Tiled

2D Median

2D Nonlinear Filter

2D Savitzky-Golay filter

Absolute Value

Anisotropic Diffusion Filter

Watershed Segmentation

Secondary Watershed Segmentation

Affine Transformation

Align Channels

Align Series (Image Stabilizer)

Align Tiled Channels

Align Tiled Series (Image Stabilizer)

Fill or Mask Active ROI

Scalar Arithmetic

Scalar Arithmetic Multi

Automatic ROI drawing

Subtract Background or Normalize

Close

Copy

Copy ROIs from Other Image

Correct Intensity Jump

Correct Lens Distortion

Plot Correlation (Colocalization)

Count Object Colocalization

Count Division and Cell Death

Create ROI

Create ROIs from Segments

Crop

Crop Image to Segments

Detect Nuclei Convolution

ΔF/F0

Differential Evolution Optimizer

Distance from Segments

Draw Model Mitochondrion

Draw Random Position Model Mitochondria

EndIf

Erase All ROIs

Excel Window Command

Export

Fill Mask

Band Pass filter Optimization

FunctionOptions

Get Image Information

Get Linked Channel

Calculate Spatial Moments

If

If Greater Than Zero

Image Arithmetic

Image Arithmetic Single Frame

Image Arithmetic In Place

Attach Intensity Gating Image

Inpaint Mask

Input

Lens Correction Optimization

Link

Load ROIs

Mask Borders

Mask Frames by Plot Values

Mask Images

Spectral Unmix

Multi-Dimensional Open Information

Multi-Dimensional Open Stage Position

Measure Object Morphology

Measure Object Intensity

2D Morphological Operator

New Image

New Time Scale

Blind Spectral Unmix with NMF

Sensor Noise Characteristics

Onset Image

Open File

Optical Flow

Options

Attach Overlay Image

Pipeline

Pipeline Optimization

Plot

Plot Intensities Corresponding to Segments

Plot Morphological Parameters of Segments

Plot Tracking Parameters

PMPI Complete Calibration

PMPI Complete Calibration with known kP

PMPI Calibration with [K+]ec steps and known [K+]ic and known kP

PMPI Short Calibration between known baseline and CDC

PMPI Short Calibration between known baseline and separately measured fP0

PMPI Short Calibration between known baseline and MDC

PMPI Short Calibration based on known potential during MDC and CDC

PMPI Short Calibration from Zero with fx=0

Potential calibration constants

Potential calibration error propagation

Potential calibration expert overrides

Calibration Wizard Parameters

T or Z-project

Projection of Vectors from a Point

Ratio

Plot Ratio

ReCount Division and Cell Death

Reevaluate Segments

Multi-Dimensional Reload Channel

Remove Blank Frames

Rename

Resample Image

ROI Classifiers

Temporal Rolling Projection

Load and Run Pipeline

Run Membrane Potential Calibration

Save ROIs

Select

Select by Number

Set Reference Image

Set Scaling/LUT

Set Segmentation Classifiers

Set Segmentation Intensity Classifiers

Calculate Simple Crossbleed

Simple 2D Cross-correlation

Simple Segmentation

Skeletonize

Strip to Well Cell Count

Substitute Poisson Noise

Template Matching

Temporal Average Filter

Temporal Block Filter

Temporal Median Filter

Temporal Savitzky-Golay Filter

Threshold

Time Stamp and Scale Bar

TMRM Complete Calibration

TMRM Complete Calibration with known kT

TMRM Short Calibration between known baseline and MDC or CDC

Track Objects

Thinness Ratio Optimization

Truncate or Cut

Wait for All Inputs

Wiener filter

Window Menu Command

Write Back Scaled Values

Cross-correlation data

Cross-correlation image

2D Kernel Convolution

2D DFT Filter

2D DFT Filter Butterworth BP

2D DFT Filter Butterworth BP Tiled

2D Median

2D Nonlinear Filter

2D Savitzky-Golay filter

Absolute Value

Anisotropic Diffusion Filter

Watershed Segmentation

Secondary Watershed Segmentation

Affine Transformation

Align Channels

Align Series (Image Stabilizer)

Align Tiled Channels

Align Tiled Series (Image Stabilizer)

Fill or Mask Active ROI

Scalar Arithmetic

Scalar Arithmetic Multi

Automatic ROI drawing

Subtract Background or Normalize

Close

Copy

Copy ROIs from Other Image

Correct Intensity Jump

Correct Lens Distortion

Plot Correlation (Colocalization)

Count Object Colocalization

Count Division and Cell Death

Create ROI

Create ROIs from Segments

Crop

Crop Image to Segments

Detect Nuclei Convolution

ΔF/F0

Differential Evolution Optimizer

Distance from Segments

Draw Model Mitochondrion

Draw Random Position Model Mitochondria

EndIf

Erase All ROIs

Excel Window Command

Export

Fill Mask

Band Pass filter Optimization

FunctionOptions

Get Image Information

Get Linked Channel

Calculate Spatial Moments

If

If Greater Than Zero

Image Arithmetic

Image Arithmetic Single Frame

Image Arithmetic In Place

Attach Intensity Gating Image

Inpaint Mask

Input

Lens Correction Optimization

Link

Load ROIs

Mask Borders

Mask Frames by Plot Values

Mask Images

Spectral Unmix

Multi-Dimensional Open Information

Multi-Dimensional Open Stage Position

Measure Object Morphology

Measure Object Intensity

2D Morphological Operator

New Image

New Time Scale

Blind Spectral Unmix with NMF

Sensor Noise Characteristics

Onset Image

Open File

Optical Flow

Options

Attach Overlay Image

Pipeline

Pipeline Optimization

Plot

Plot Intensities Corresponding to Segments

Plot Morphological Parameters of Segments

Plot Tracking Parameters

PMPI Complete Calibration

PMPI Complete Calibration with known kP

PMPI Calibration with [K+]ec steps and known [K+]ic and known kP

PMPI Short Calibration between known baseline and CDC

PMPI Short Calibration between known baseline and separately measured fP0

PMPI Short Calibration between known baseline and MDC

PMPI Short Calibration based on known potential during MDC and CDC

PMPI Short Calibration from Zero with fx=0

Potential calibration constants

Potential calibration error propagation

Potential calibration expert overrides

Calibration Wizard Parameters

T or Z-project

Projection of Vectors from a Point

Ratio

Plot Ratio

ReCount Division and Cell Death

Reevaluate Segments

Multi-Dimensional Reload Channel

Remove Blank Frames

Rename

Resample Image

ROI Classifiers

Temporal Rolling Projection

Load and Run Pipeline

Run Membrane Potential Calibration

Save ROIs

Select

Select by Number

Set Reference Image

Set Scaling/LUT

Set Segmentation Classifiers

Set Segmentation Intensity Classifiers

Calculate Simple Crossbleed

Simple 2D Cross-correlation

Simple Segmentation

Skeletonize

Strip to Well Cell Count

Substitute Poisson Noise

Template Matching

Temporal Average Filter

Temporal Block Filter

Temporal Median Filter

Temporal Savitzky-Golay Filter

Threshold

Time Stamp and Scale Bar

TMRM Complete Calibration

TMRM Complete Calibration with known kT

TMRM Short Calibration between known baseline and MDC or CDC

Track Objects

Thinness Ratio Optimization

Truncate or Cut

Wait for All Inputs

Wiener filter

Window Menu Command

Write Back Scaled Values

Cross-correlation data

Cross-correlation image

Image Analyst MKII Functions

Name |
Short Name |
Type |
Description |

Name of the parameter | Short name used when called from
Mathematica |
integer, real, bool, string |

Each image processing
function has an input of one or more image sequences, and a set of
parameters shown in the above table.

Image processing functions in Image Analyst MKII can be accessed from the Main Menu items between "I/O" and "Special".

These functions are also accessible from the Pipeline.

All functions are accessible in a fully parametric form from* Wolfram Research* *Mathematica*.
'Short Names' are used when calling functions from *Mathematica*.

Select a function on the Right -------------->

Image processing functions in Image Analyst MKII can be accessed from the Main Menu items between "I/O" and "Special".

These functions are also accessible from the Pipeline.

All functions are accessible in a fully parametric form from

Select a function on the Right -------------->