2D DFT Filter
2D DFT Filter Butterworth BP
2D DFT Filter Butterworth BP Tiled
2D Kernel Convolution
2D Median
2D Morphological Operator
2D Nonlinear Filter
2D Savitzky-Golay filter
Absolute Value
Affine Transformation
AI Create Training Set
Align Channels
Align Series (Image Stabilizer)
Align Tiled Channels
Align Tiled Series (Image Stabilizer)
Anisotropic Diffusion Filter
Attach Intensity Gating Image
Attach Overlay Image
Automatic ROI drawing
Band Pass filter Optimization
Blind Spectral Unmix with NMF
Brightfield Color Deconvolution with PCA
Calculate Simple Crossbleed
Calculate Spatial Moments
Calibration Wizard Parameters
Clear Segmentation Classifiers (Gating)
Close
Copy
Copy ROIs from Other Image
Correct Intensity Jump
Correct Lens Distortion
Count Division and Cell Death
Count Object Colocalization
Create ROI
Create ROI Grid
Create ROIs from Segments
Create Segments from ROIs
Crop
Crop Image in Place
Crop Image to Segments
Cross-correlation data
Cross-correlation image
Detect Nuclei Convolution
Differential Evolution Optimizer
Distance from Segments
Draw Model Mitochondrion
Draw Random Position Model Mitochondria
EndIf
Erase All ROIs
Excel Window Command
Export
Fill Mask
Fill or Mask Active ROI
Find ROIs and Annotations
Fix Vignetting
FLIPR Calibration with [K+]ec steps and known [K+]ic and known kP
FLIPR Complete Calibration
FLIPR Complete Calibration with known kP
FLIPR Complete Calibration with known kP - Goldman
FLIPR Complete Calibration with known kP - Goldman with Neural Network
FLIPR Complete Iterative Calibration
FLIPR Estimate PN
FLIPR Short Calibration based on known potential during MDC and CDC
FLIPR Short Calibration between known baseline and CDC
FLIPR Short Calibration between known baseline and MDC
FLIPR Short Calibration between known baseline and separately measured fP0
FLIPR Short Calibration from Zero with fx=0
FunctionOptions
Get Image Information
Get Linked Channel
If
If Greater Than Zero
Image Arithmetic (image-image math)
Image Arithmetic In Place
Image Arithmetic Single Frame
Inpaint Mask
Input
Invert
Ion Concentration Calibration Constants
Ion Concentration Calibration Error Propagation
Ion Concentration Calibration Overrides
Ion Concentration Calibration Wizard Parameters
Lens Correction Optimization
Link
Load and Run Pipeline
Load ROIs
Mask Borders
Mask Frames by Plot Values
Mask Images
Measure Object Intensity
Measure Object Morphology
Mirror or Rotate
Mirror or Rotate in Place (clipping)
Model Out of Focus Blur
Multi-Dimensional Open Information
Multi-Dimensional Open Position
Multi-Dimensional Reload Channel
New Image
New Time Scale
Onset Image
Open File
Optical Flow
Options
Peak Bioenergetic Power Analysis
Pipeline
Pipeline Optimization
Pipeline Optimization Parameter
Plot
Plot Correlation (Colocalization)
Plot Intensities Corresponding to Segments
Plot Morphological Parameters of Segments
Plot Ratio
Plot ROI Dimensions
Plot Spectral Parameter with PCA
Plot Spectrum (Periodogram)
Plot Tracking Parameters
Potential calibration constants
Potential calibration error propagation
Potential calibration expert overrides
Projection of Vectors from a Point
Ratio
Ratiometric Ion Concentration with Grynkiewicz Equation - Complete Internal
Ratiometric ROI Classifiers (Gating)
ReCount Division and Cell Death
Reevaluate Segments (Apply Gate)
Remove Blank or Unsharp Frames
Rename
Replace or Modify ROIs
Resample Image
Resize Image
Revvity Harmony Options
ROI Classifiers (Gating)
Run EXE
Run Membrane Potential Calibration
Save ROIs
Scalar Arithmetic (image-value math)
Scalar Arithmetic Multi
Secondary Watershed Segmentation
Select
Select by Number
Sensor Noise Characteristics
Set Reference Image
Set ROI Annotation
Set Scaling/LUT
Set Segmentation Classifiers (Gating)
Set Segmentation Intensity Classifiers (Gating)
Shift Time Scale
Simple 2D Cross-correlation
Simple Segmentation
Skeletonize
Spectral Unmix
Stitch Series
Strip to Well Cell Count
Substitute Poisson Noise
Subtract Background or Normalize
T or Z-project
Template Matching
Temporal Average Filter
Temporal Block Filter
Temporal Median Filter
Temporal Rolling Projection
Temporal Savitzky-Golay Filter
Thinness Ratio Optimization
Threshold
Time Stamp and Scale Bar
TMRM Complete Calibration
TMRM Complete Calibration with known kT
TMRM Complete Calibration with known kT and K-steps
TMRM Short Calibration between known baseline and MDC or CDC
Track Objects
Truncate or Cut
Wait for All Inputs
Watershed Segmentation
Wiener filter
Window Menu Command
Write Back Scaled Values
ΔF/F0

Lens Correction Optimization ( IALensDistCorrectionOptimize )

Parameters:

Name	Short Name	Type	Description
Type of correction	type	string
Number of tiles in x	tilesx	integer	The image consists of this number of equal sized tiles in x dimension.
Number of tiles in y	tilesy	integer	The image consists of this number of equal sized tiles in y dimension.
Min Focal length along the x axis	fxMin	real
Max Focal length along the x axis	fxMax	real
Min Focal length along the y axis	fyMin	real
Max Focal length along the y axis	fyMax	real
Min x-coordinate of the principal points	cxMin	real	This is a coordinate in pixels
Max x-coordinate of the principal points	cxMax	real	This is a coordinate in pixels
Min y-coordinate of the principal point	cyMin	real	This is a coordinate in pixels
Max y-coordinate of the principal point	cyMax	real	This is a coordinate in pixels
Min First coefficient of radial distortion	k1Min	real
Max First coefficient of radial distortion	k1Max	real
Min Second coefficient of radial distortion	k2Min	real
Max Second coefficient of radial distortion	k2Max	real
Min First coefficient of tangential distortion	p1Min	real
Max First coefficient of tangential distortion	p1Max	real
Min Second coefficient of tangential distortion	p2Min	real
Max Second coefficient of tangential distortion	p2Max	real

Description:

Optimization of lens distortion parameters. Select image to undistort as "Image" to align it to another image selected as "Mask". Typically "Image" and "Mask" show the same fluorescent structures, but recorded at different emission wavelengths.
"Image" and "Mask" are e.g. red and green channels, and the red is substantially distorted because of the chromatic/spherical aberration of the lens.
Select at "type of correction" which parameters are to be optimized and set the range of optimization below.