Correct Lens Distortion ( IACorrectLensDistortion )

Parameters:

Name	Short Name	Type	Description
Number of tiles in x	tilesx	integer	The image consists of this number of equal sized tiles in x dimension.
Number of tiles in y	tilesy	integer	The image consists of this number of equal sized tiles in y dimension.
Focal length along the x axis	fx	real	The focal length is in pixels; the lens working distance (um) divided by image resolution (um/pixels).
Focal length along the y axis	fy	real	The focal length is in pixels; the lens working distance (um) divided by image resolution (um/pixels).
x-coordinate of the principal point	cx	real	This is a coordinate in pixels, if the camera view field was properly centered, then the half of the image (tile) size.
y-coordinate of the principal point	cy	real	This is a coordinate in pixels, if the camera view field was properly centered, then the half of the image (tile) size.
First coefficient of radial distortion	k1	real	Positive value pulls sides towards the center, negative value pushes away. Try values around +/- 1.
Second coefficient of radial distortion	k2	real	Positive value pulls sides towards the center, negative value pushes away. Try values around +/- 1.
First coefficient of tangential distortion	p1	real	Typically zero for microscopy.
Second coefficient of tangential distortion	p2	real	Typically zero for microscopy.

Description:

Corrects lens spherical aberration in a single channel. Use this function for more precise colocalization measurements, when chromatic aberration of the lens prevents proper overlay of images recorded at very different emission wavelength.
To obtain parameters of this function use the built-in optimization algorithm, that undistorts one image to match (for best overlay) another. To access the optimization algorithm, go "Tools/Setup DFT filter and Optimizations",
in the "Optimization" tab, "Select parameters of" choose "Lens Distortion Correction Parameters".
The image can be tiled and the transformation is performed in each tile frame. If no tiling used, set 1 for number of tiles.
Focal length is a positive number and given in pixels, so its value is around the lens working distance (um) divided by image resolution (um/pixels).
The principal point is the position in the image where the distortion is zero, given in pixels. If the lens is perfectly centered, then the middle of the image, so half of the image size.
Coefficients of radial distortion: positive value pulls sides towards the center, negative value pushes away. Try values around +/- 1.