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FLIPR Short Calibration from Zero with fx=0
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ΔF/F0

FLIPR Short Calibration based on known potential during MDC and CDC ( IAFLIPRShortMDCCDC )

Parameters:

Name	Short Name	Type	Description
Range definitions in time courses	rangedef	string	Defines whether the ranges given below refer to frames or seconds in the recordings
Baseline Range	BaselineRange	string	Defines the range of the baseline (in the above defined time units). Longer baseline results more accurate calibration.
MDC Range	MDCRange	string	The range of Mitochondrial Depolarization Cocktail addition (in the above defined time units).
ΔψP at MDC or Keq (mV)	PMP1	real	Previously measured value of ΔψP during Mitochondrial Depolarization Cocktail treatment.
CDC Range	CDCRange	string	The range of Complete Depolarization Cocktail addition (in the above defined time units).
kP	kP	real	Previously measured value of kp
fP0-fPX Allowed Range	fP0MinusfPXRange	string	Trace selection criterion: the minimum and maximum allowed value of fP0-fPX
Take maximum of CDC Range	CDCMax	boolean	If set yes, the maximum FLIPR intensity within the CDC Range is taken as zero calibration value (it error is the local SD of the range). Otherwise mean is calculated.

Description:

This method calculates plasma membrane potential (ΔψP) time course based on previously measured potential during MDC, zero potential in the presence of CDC and kP using the FLIPR Membrane Potential Kit from Molecular Devices (aka PMPI, plasma membrane potential indicator).
Required experimental protocol: any time lapse, followed by establishment of K-equilibrium potential and finally complete depolarization.