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FLIPR Short Calibration from Zero with fx=0
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ΔF/F0

FLIPR Short Calibration from Zero with fx=0 ( IAFLIPRShortZero )

Parameters:

Name	Short Name	Type	Description
Range definitions in time courses	rangedef	string	Defines whether the ranges given below refer to frames or seconds in the recordings
Baseline Range	BaselineRange	string	Defines the range of the baseline (in the above defined time units). Longer baseline results more accurate calibration.
CDC Range	CDCRange	string	The range of Complete Depolarization Cocktail addition (in the above defined time units).
kP	kP	real	Previously measured value of kp
Take maximum of CDC Range	CDCMax	boolean	If set yes, the maximum FLIPR intensity within the CDC Range is taken as zero calibration value (it error is the local SD of the range). Otherwise mean is calculated.

Description:

This method calculates resting plasma membrane potential (ΔψP) and time course based on the fluorescence at zero potential using the FLIPR Membrane Potential Kit from Molecular Devices (aka PMPI, plasma membrane potential indicator).
Required experimental protocol: any time lapse and finally complete depolarization. The fluorescence background is assumed to be zero.