1) Background removal with high pass filtering
2) Organelle motion assay with Optical Flow
3) Image acquisition for mitochondrial motion in Elements and analysis in Image Analyst MKII
4) Image acquisition for mitochondrial motion in Metamorph and analysis in Image Analyst MKII
5) Image acquisition for mitochondrial motion in Zeiss LSM and analysis in Image Analyst MKII
6) Working with Optical Flow images
7) Fine Tuning Optical Flow
8) Measurement of velocities and fluorescence intensities over single mitochondria
9) Measurements of morphological properties of mitochondria e.g. length or numbers using built-in pipelines
10) Measurements of mitochondrial morphological properties, velocities and intensities in populations or individual mitochondria
11) Mitochondrial swelling measurement with "ratiometric optimized spatial filtering technique" AKA "thinness ratio"
12) Measurement of mitochondria:cell volume fraction (VF)
13) Measurement of apparent activity coefficient ratio for TMRM (aR')
14) Absolute mitochondrial membrane potential measurement in (beta-)cells
15) Calculation of spectral unmixing coefficients for mitochondrial membrane potential measurement
16) Reactive oxygen species assay with normalized rate of DCFDA oxidation
17) Assay of levels of reactive oxygen species using DHE ratioing
18) Assay of mitochondrial levels of reactive oxygen species using OH-ethidium rate measurement and MitoSOX:TMRM:MitoTracker Deep Red double normalization
19) Assay of mitochondrial levels of reactive oxygen species using MitoSOX:MitoTracker Deep red end point measurement
ProtocolsProtocols
