Image Analyst MKIIQuick How To
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Quick How To

How to subtract background?

How to measure and save fluorescence intensity values using ROIs?

How to run Image Stabilizer (register or align images)

How to create and use Reference Images?

How to run a pipeline?

How to work with XYZT recordings (time lapse of z-stacks)?

How to work with recordings from multiwell plates?

When to use the internal loader and when Bio-Formats?

How to scale image intensities identically for comparison?

How to transfer images to a presentation software?

How to overlay images?

How to segment an image (series) showing fluorescent cells or nuclei?

How to count cells in view fields, whole wells or whole microplates?

How to measure rates of change of fluorescence intensity?

How to calculate fluorescence ratio?

How to apply high pass filtering to selectively measure mitochondrial fluorescence?