Measure fluorescence ratio of whole view field with background masking

Parameters:

Name	#	Type	Description
Channel Number (Numerator)	1	integer	Channel number for the numerator in the fluorescence ratio calculation
Channel Number (Denominator)	2	integer	Channel number for the denominator in the fluorescence ratio calculation
Background Level (Percentile)	3	real	Background is calculated as frame-by-frame mean of those pixels that are the darker than this percentile of image histogram for the duration of the entire recording.
Mask sensitivity (0.2-1.5)	4	real	Sensitivity factor, typically 0.2-1.5
Plot normalization	5	string	This option supersedes ΔF/F0 normalization.
Show results in microplate format	6	boolean	Organizes data in table according to microplate well associations in additional worksheets.

Description:

Calculates average fluorescence ratio of all cells in the view field after stabilization of images, background subtraction and masking of background regions. The mask is calculated from maximum intensity projection images therefore accounts for cellular movement during the timelapse. The fluorescence ratio is calculated as the ratio of mean fluorescence intensities allowing working at low signal to noise ratio. This pipeline is ideal for obtaining fluorescence ratio from low-light level recordings, where cellular movement occurs. Applicable to Ca2+-imaging (e.g. Fura-2, yellow cameleons), other ratiometric ion probes imaging (e.g. PBFI, SPFI, SNARF-1, BCECF), reactive oxygen species imaging (e.g. HyPer, roGFP), and intramolecular FRET sensor imaging.

Version history:
V2
Changed plotting property: Place channels into columns
Added "Show results in microplate format" option.