Image Analyst MKIIWorkflow
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>Open Image sequences

>Multi-Dimensional data sets

>Image Window

>Set Image Linkage

> Parametering image processing functions

>Dialogs

>>Multi-Dimensional Open

>>Setup DFT Filter

>>Attach IMD Intensity

>>Attach Overlay Image

>>Attach Overlay Text

>>Pipeline Optimizer

>>Membrane Potential Calibration Wizard

>>Interactive Ion Calibration

>>Crossbleed correction

>>Preferences

>Fixing imaging artifacts

>Smoothing

>Pipeline

>Mathematica

>Annotate, overlay

>Define ROIs

>Background subtraction

>Plotting intensities, ratios

>Masking images

>Thresholding

>Segmenting images

>Plot morphological parameters

>Save or copy plot data in tabular format

>Export RGB images or movies

>Evaluate expressions

Workflow in Image Analyst MKII
 

1) Open Open Image sequences of one or more gray value channels load
Multi-Dimensionaldata sets and Bio-formats load
Set Image Linkage link
2) Process Parametering and running image processing functions and pipelines Function
Dialogs dialogs
Fixing imaging artifacts IS
Smoothing and optimizing the look of images  
Automation of processing using Pipelines Pipeline
Using Mathematica as scripting language  
Annotate, overlay 00:00:00
3) Quantify Define ROIs roi
Subtract background, normalize, OD background subtraction
Plot intensities, ratios of intensities and Excel Data Window plot
Masking images MASK
Thresholding and Segmenting images  
Plot morphological parameters plot
4) Export Save or copy plot data in tabular format save plot data
Export RGB images or movies export