Opening an experiment results one or more image windows (according to the number of channels or wavelengths) with identical x,y,t dimensions. These image windows are opened as being linked together, and this linkage enables processing involving two or more of these image sequences, like image arithmetic, spectral unmixing or overlying. Image Analyst MKII does not limit the number of experiments or opened image windows.
To perform image processing maneuvers requiring multiple inputs, image windows must be linked. This rule can be violated using Pipeline, but it has to be kept in mind that most image processing functions require identical x,y,t dimensions between the input images. Some image processing functions that require different sized inputs allow selecting non-linked Image Windows as 'Image B'
Multiple sets of linked image windows can be present simultaneously. Switch between linked image windows by right click over an image window and selecting Switch to window. Or use the Window menu.
Linked image windows share ROIs, therefore drawing, moving deleting a ROI in any of the linked set of image windows affects all of them.
When image windows are unlinked ROIs are copied.
When new image windows are linked ROIs are merged.
To link or unlink image windows click Edit/Set Linkage in the Main Menu or the toolbar button. From pipelines use the I/O/Link function.