Image Window

Image window contains a time lapse of frames. Each pixel is stored as a 32bit floating point number, thus has a numeric value between ±3.4´10³⁸ or as little as 1.2´10^-38. Pixels may have no numeric values, these pixels are considered as masked. Actually, masking happens by setting pixels to non-numeric values, e.g. by division by zero, using division by a binarized image.
The Image Window scales floating point data to grayscale intensities or a palette of colors (look up table, LUT), as indicated by the Intensity scale bar. Importantly, intensity scaling and the LUT are only for visualization, and the actual image data in the memory is not affected by the intensity scaling. Therefore a distinction has to be made between the grayscale or RGB image seen in the Image Window, and the complete 32bit data set in the memory. All image processing functions and plot generation operate on the data stored in the memory, except for the

Write Back Scaled Values function, which stores the visualized images as image data in the memory. When an image window is copied to the clipboard

or exported to file

, the visualized image in RGB format is outputted. When an image window is duplicated

, the complete 32bit data set is duplicated.

To set the look up table (LUT) or the intensity scaling use right click on the image window and the LUT and Scaling menu points. Alternatively drag the sides of the scale bar in the bottom of the Image Window The gamma value can be set in the LUT menu or by shift-left click and dragging the middle of the Intensity scale bar.

To draw ROIs use the following toolbar buttons: point ROI:

line ROI:

area ROI:

magic wand tool:

. Set parameters of the magic wand tool in the Edit/Preferences dialog.
To select and move ROIs use

in the toolbar. Use the Ctrl key to toggle between drawing or moving ROIs.
To erase selected ROI use DEL, the context menu of the Image Window or

in the toolbar. ROIs can be also deleted by the selecting and deleting a trace using the DEL key in a Plot Window.
ROIs cannot be modified, just repositioned one finished drawing.
ROIs are shared between linked Image Windows.

Time line:
use scroll or drag mouse to move. Use left and right arrows on keyboard for on frame steps
	(x,y) position of pointer pixel value under the pointer position on the timescale in s and in (frame/total frames) ROIs: number of ROIs, and the active ROI in brackets.	Intensity scale bar Click on minimum or maximum values ad drag them horizontally with the left mouse button down to scale Drag the middle to scale both values Shift-left click and drag the middle to set gamma value

Use the

button in the toolbar to play image sequence. Set the frame rate in the Edit/Preferences dialog.

Use the

button in the toolbar to synchronize the frame number shown in all linked image windows.

Double-click the status bar for image properties.

The context menu (right click menu) of the Image Window

The following functions are available in the context menu of the image window, and some of them also as image processing functions in the main menu:

Process this... :if a single operand function is selected, the content of the Image Window is processed. For multiple operand functions a check mark appears here whether to include the Image Window as operand.
Zoom (alternatively use resizing the window to zoom, or the scroller of the mouse or double click when the ROI tool is selected in the main toolbar.)
Look Up Table (LUT) selection (see also Playback/Set Scaling/LUT)
Set scaling (see also Playback/Set Scaling/LUT)
Attach overlay, intensity modulated display (IMD), and annotation (see also Playback/Attach... functions)
Invoke image information: this text window contains metadata that was recorded by the image acquisition software
ROI and clipboard functions (see also in the toolbar and in the Edit Menu)