Image Analyst MKIIWorkflow
HomeWorkflowImage Processing PrinciplesFunctions GlossaryPipelines GlossaryProtocolsQuick How ToSearch

Attach Intensity Gating (IMD Intensity) , Attach Overlay Image, Attach Overlay Text

Attach Intensity Gating

Intensity gating or Intensity Modulated Display (IMD) is a special Look Up Table (LUT), which is used for visualization of ratios (or other derived parameters) only where significant intensity, thus information is in the original image (from which the ratio or derived parameter was calculated). Therefore the pixel values determine the hue (the color), and the attached IMD intensity image determines the brightness of the pixel. The attached image is typically the one before ratio calculation, but the function allows to use any other images, so images can be filtered, denoised, high pass filtered before attachment as IMD intensity. The attached image has to be use a grayscale LUT. Intensity Modulated Display is a a LUT operation, so the image data is not affected, only the visualization.

Synchronized: the same frame of the time lapse used.
Frozen: the frame used as intensity image
Show non linked: all open images with same x,y dimensions are listed, but non-linked images can be attached only as frozen, because their frame number is not necessarily identical.

Attach Overlay Image:

RGB overlay of selected images; the image data is not affected, only the visualization.

Synchronized: the same frame of the time lapse used.
Frozen: the frame used as intensity image
Show non linked: all open images with same x,y dimensions are listed, but non-linked images can be attached only as frozen, because their frame number is not necessarily identical.

Attach Overlay Text:
Image annotation tool to show scale bar, frame number and time point in the image.

See also:
These functionality of these dialogs are also available as functions:
FAttach Intensity Gating
FAttach Overlay Image
FAttach Overlay Text

Overlay and annotation are exported or copied to the clipboard with RGB images, but it does not affect image data. It does not affect image data even when using the FWrite Back Scaled Values function.