2D DFT Filter

2D DFT Filter Butterworth BP

2D DFT Filter Butterworth BP Tiled

2D Kernel Convolution

2D Median

2D Morphological Operator

2D Nonlinear Filter

2D Savitzky-Golay filter

Absolute Value

Affine Transformation

Align Channels

Align Series (Image Stabilizer)

Align Tiled Channels

Align Tiled Series (Image Stabilizer)

Anisotropic Diffusion Filter

Attach Intensity Gating Image

Attach Overlay Image

Automatic ROI drawing

Band Pass filter Optimization

Blind Spectral Unmix with NMF

Calculate Simple Crossbleed

Calculate Spatial Moments

Calibration Wizard Parameters

Clear Segmentation Classifiers

Close Image Window

Copy Image Window

Copy ROIs from Other Image

Correct Intensity Jump

Correct Lens Distortion

Count Division and Cell Death

Count Object Colocalization

Create ROI

Create ROIs from Segments

Crop

Crop Image in Place

Crop Image to Segments

Cross-correlation data

Cross-correlation image

Detect Nuclei Convolution

Differential Evolution Optimizer

Distance from Segments

Draw Model Mitochondrion

Draw Random Position Model Mitochondria

EndIf

Erase All ROIs

Excel Window Command

Export

Fill Mask

Fill or Mask Active ROI

FLIPR Calibration with [K+]ec steps and known [K+]ic and known kP

FLIPR Complete Calibration

FLIPR Complete Calibration with known kP

FLIPR Complete Calibration with known kP - Goldman

FLIPR Complete Iterative Calibration

FLIPR Estimate PN

FLIPR Short Calibration based on known potential during MDC and CDC

FLIPR Short Calibration between known baseline and CDC

FLIPR Short Calibration between known baseline and MDC

FLIPR Short Calibration between known baseline and separately measured fP0

FLIPR Short Calibration from Zero with fx=0

FunctionOptions

Get Image Information

Get Linked Channel

If

If Greater Than Zero

Image Arithmetic (image-image math)

Image Arithmetic In Place

Image Arithmetic Single Frame

Inpaint Mask

Input

Invert

Lens Correction Optimization

Link Image Windows

Load and Run Pipeline

Load ROIs

Mask Borders

Mask Frames by Plot Values

Mask Images

Measure Object Intensity

Measure Object Morphology

Mirror or Rotate (new image)

Mirror or Rotate in Place

Multi-Dimensional Open Information

Multi-Dimensional Open Stage Position

Multi-Dimensional Reload Channel

New Image

New Time Scale

Onset Image

Open File

Optical Flow

Options

Pipeline

Pipeline Optimization

Pipeline Optimization Parameter

Plot

Plot Correlation (Colocalization)

Plot Intensities Corresponding to Segments

Plot Morphological Parameters of Segments

Plot Ratio

Plot ROI Dimensions

Plot Tracking Parameters

Potential calibration constants

Potential calibration error propagation

Potential calibration expert overrides

Projection of Vectors from a Point

Ratio

Ratiometric ROI Classifiers

ReCount Division and Cell Death

Reevaluate Segments

Remove Blank Frames

Rename

Resample Image

ROI Classifiers

Run Membrane Potential Calibration

Save ROIs

Scalar Arithmetic (image-value math)

Scalar Arithmetic Multi

Secondary Watershed Segmentation

Select

Select by Number

Sensor Noise Characteristics

Set Reference Image

Set Scaling/LUT

Set Segmentation Classifiers

Set Segmentation Intensity Classifiers

Shift Time Scale

Simple 2D Cross-correlation

Simple Segmentation

Skeletonize

Spectral Unmix

Strip to Well Cell Count

Substitute Poisson Noise

Subtract Background or Normalize

T or Z-project

Template Matching

Temporal Average Filter

Temporal Block Filter

Temporal Median Filter

Temporal Rolling Projection

Temporal Savitzky-Golay Filter

Thinness Ratio Optimization

Threshold

Time Stamp and Scale Bar

TMRM Complete Calibration

TMRM Complete Calibration with known kT

TMRM Complete Calibration with known kT and K-steps

TMRM Short Calibration between known baseline and MDC or CDC

Track Objects

Truncate or Cut

Wait for All Inputs

Watershed Segmentation

Wiener filter

Window Menu Command

Write Back Scaled Values

ΔF/F0

2D DFT Filter Butterworth BP

2D DFT Filter Butterworth BP Tiled

2D Kernel Convolution

2D Median

2D Morphological Operator

2D Nonlinear Filter

2D Savitzky-Golay filter

Absolute Value

Affine Transformation

Align Channels

Align Series (Image Stabilizer)

Align Tiled Channels

Align Tiled Series (Image Stabilizer)

Anisotropic Diffusion Filter

Attach Intensity Gating Image

Attach Overlay Image

Automatic ROI drawing

Band Pass filter Optimization

Blind Spectral Unmix with NMF

Calculate Simple Crossbleed

Calculate Spatial Moments

Calibration Wizard Parameters

Clear Segmentation Classifiers

Close Image Window

Copy Image Window

Copy ROIs from Other Image

Correct Intensity Jump

Correct Lens Distortion

Count Division and Cell Death

Count Object Colocalization

Create ROI

Create ROIs from Segments

Crop

Crop Image in Place

Crop Image to Segments

Cross-correlation data

Cross-correlation image

Detect Nuclei Convolution

Differential Evolution Optimizer

Distance from Segments

Draw Model Mitochondrion

Draw Random Position Model Mitochondria

EndIf

Erase All ROIs

Excel Window Command

Export

Fill Mask

Fill or Mask Active ROI

FLIPR Calibration with [K+]ec steps and known [K+]ic and known kP

FLIPR Complete Calibration

FLIPR Complete Calibration with known kP

FLIPR Complete Calibration with known kP - Goldman

FLIPR Complete Iterative Calibration

FLIPR Estimate PN

FLIPR Short Calibration based on known potential during MDC and CDC

FLIPR Short Calibration between known baseline and CDC

FLIPR Short Calibration between known baseline and MDC

FLIPR Short Calibration between known baseline and separately measured fP0

FLIPR Short Calibration from Zero with fx=0

FunctionOptions

Get Image Information

Get Linked Channel

If

If Greater Than Zero

Image Arithmetic (image-image math)

Image Arithmetic In Place

Image Arithmetic Single Frame

Inpaint Mask

Input

Invert

Lens Correction Optimization

Link Image Windows

Load and Run Pipeline

Load ROIs

Mask Borders

Mask Frames by Plot Values

Mask Images

Measure Object Intensity

Measure Object Morphology

Mirror or Rotate (new image)

Mirror or Rotate in Place

Multi-Dimensional Open Information

Multi-Dimensional Open Stage Position

Multi-Dimensional Reload Channel

New Image

New Time Scale

Onset Image

Open File

Optical Flow

Options

Pipeline

Pipeline Optimization

Pipeline Optimization Parameter

Plot

Plot Correlation (Colocalization)

Plot Intensities Corresponding to Segments

Plot Morphological Parameters of Segments

Plot Ratio

Plot ROI Dimensions

Plot Tracking Parameters

Potential calibration constants

Potential calibration error propagation

Potential calibration expert overrides

Projection of Vectors from a Point

Ratio

Ratiometric ROI Classifiers

ReCount Division and Cell Death

Reevaluate Segments

Remove Blank Frames

Rename

Resample Image

ROI Classifiers

Run Membrane Potential Calibration

Save ROIs

Scalar Arithmetic (image-value math)

Scalar Arithmetic Multi

Secondary Watershed Segmentation

Select

Select by Number

Sensor Noise Characteristics

Set Reference Image

Set Scaling/LUT

Set Segmentation Classifiers

Set Segmentation Intensity Classifiers

Shift Time Scale

Simple 2D Cross-correlation

Simple Segmentation

Skeletonize

Spectral Unmix

Strip to Well Cell Count

Substitute Poisson Noise

Subtract Background or Normalize

T or Z-project

Template Matching

Temporal Average Filter

Temporal Block Filter

Temporal Median Filter

Temporal Rolling Projection

Temporal Savitzky-Golay Filter

Thinness Ratio Optimization

Threshold

Time Stamp and Scale Bar

TMRM Complete Calibration

TMRM Complete Calibration with known kT

TMRM Complete Calibration with known kT and K-steps

TMRM Short Calibration between known baseline and MDC or CDC

Track Objects

Truncate or Cut

Wait for All Inputs

Watershed Segmentation

Wiener filter

Window Menu Command

Write Back Scaled Values

ΔF/F0

Write Back Scaled Values ( IAWriteBack )

Name |
Short Name |
Type |
Description |

Range | range | real | Range of intensities in resultant image [0-value] |

Clip range boundaries | clip | boolean | Bottoms at zero and ceilings at range value. When gamma<>1 value is used, zero clipping always occur. |

Replaces the image data with the scaled (visualized) values, set by the scaling and gamma correction of the image.

The output is bottomed at zero and ceilinged at Range value if the "Clip range boundaries" is enabled. If the image is scaled with a gamma value other than 1, zero clipping will performed, and only the ceiling clipping is optional.

The output is bottomed at zero and ceilinged at Range value if the "Clip range boundaries" is enabled. If the image is scaled with a gamma value other than 1, zero clipping will performed, and only the ceiling clipping is optional.