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ΔF/F0

TMRM Short Calibration between known baseline and MDC or CDC ( IATMRMShortBaselineMDCorCDC )

Parameters:

Name	Short Name	Type	Description
Range definitions in time courses	rangedef	string	Defines whether the ranges given below refer to frames or seconds in the recordings
Baseline Range	BaselineRange	string	Defines the range of the baseline (in the above defined time units). Longer baseline results more accurate calibration.
VF (mitochondria:cell volume fraction)	VF	real	Confocal stereologic volume fraction of mitochondria (inner boundary membrane) to whole cell (including nucleus)
VFM (matrix:mitochondrion volume fraction)	VFM	real	EM stereologic volume fraction of matrix to mitochondrion (inner boundary membrane)
aR` (matrix:cytosol apparent activity coefficient ratio)	aR	real	Apparent activity coefficient ratio measured by confocal microscopy
ΔψM at baseline (mV)	MMP0	real	Previously measured value of ΔψM at baseline.
MDC/CDC Range	MDCRange	string	The complete range of Mitochondrial and/or Complete Depolarization Cocktail addition (in the above defined time units). Ideally a range of consecutive additions of MDC and CDC are selected.
Assume equilibrium for MDC/CDC Range	AssumeEquilibrium	boolean	Assumes equilibrium, thus uses DT=0.
kT	kT	real	Previously measured value of kT
fT0-fTX Allowed Range	fT0MinusfTXRange	string	Trace selection criterion: the minimum and maximum allowed value of fT0-fTX
Quality control based on MDC calibrates to zero	QCMDC	boolean	Removes traces where MDC does not calibrate to zero, or noisy. Set threshold below.
Maximum deviation from zero (mV)	QCMDCdeviation	real	Traces where the absolute mean or SD of MDC Range is larger than this number are removed.

Description:

This method calculates mitochondrial membrane potential (ΔψM) time course based on previously known baseline potential and kP. The zero point of the calibration is MDC or CDC.
Required experimental protocol: baseline, any time lapse, followed by mitochondrial or complete depolarization,
If the baseline is not steady, use the "Do not assume equilibrium baseline" switch in the Constants tab.