Detect Nuclei Convolution ( IADetectNucleiConvolution )
Parameters:
| Name |
Short Name |
Type |
Description |
| Way |
way |
string |
Select whether discs to be detected are brighter or darker than the surrounding areas |
| Inner diameter |
id |
integer |
Diameter of the nucleus in pixels |
| Outer diameter |
od |
integer |
Diameter of the cell in pixels |
Description:
Convolves the image with a disc shaped kernel, that contains:
When "Way" is "Dark inside": an "Outer diameter" disc of positive coefficients with an "Inner diameter" disc of negative coefficients in the middle.
When "Way" is "Bright inside": an "Outer diameter" disc of negative coefficients with an "Inner diameter" disc of positive coefficients in the middle.
Set "Inner diameter" to the approximate nucleus size and "Outer diameter" to the approximate cell size. Use Watershed Segmentation following this function.
Functions
Glossary
