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FLIPR Calibration with [K+]ec steps and known [K+]ic and known kP
FLIPR Complete Calibration
FLIPR Complete Calibration with known kP
FLIPR Complete Calibration with known kP - Goldman
FLIPR Complete Calibration with known kP - Goldman with Neural Network
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FLIPR Estimate PN
FLIPR Short Calibration based on known potential during MDC and CDC
FLIPR Short Calibration between known baseline and CDC
FLIPR Short Calibration between known baseline and MDC
FLIPR Short Calibration between known baseline and separately measured fP0
FLIPR Short Calibration from Zero with fx=0
FunctionOptions
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ΔF/F0

FLIPR Estimate PN ( IAFLIPREstimatePN )

Parameters:

Name	Short Name	Type	Description
Range definitions in time courses	rangedef	string	Defines whether the ranges given below refer to frames or seconds in the recordings
Baseline Range	BaselineRange	string	Defines the range of the baseline (in the above defined time units). Longer baseline results more accurate calibration.
CDC Range	CDCRange	string	The range of Complete Depolarization Cocktail addition (in the above defined time units).
ΔψP at baseline (mV)	PMP0	real	Previously measured value of ΔψP at baseline.
kP	kP	real	Previously measured value of kp
fP0-fPX Allowed Range	fP0MinusfPXRange	string	Trace selection criterion: the minimum and maximum allowed value of fP0-fPX
Take maximum of CDC Range	CDCMax	boolean	If set yes, the maximum FLIPR intensity within the CDC Range is taken as zero calibration value (it error is the local SD of the range). Otherwise mean is calculated.
K+ Steps Ranges	KStepsRanges	string	Enter a list of range definitions for the duration of the K+-steps (in the above defined time units), including the MDC treatment.
[K+]ec Steps (mM;mM)	KStepsConcentrations	string	Extracellular (ec) K+ concentrations corresponding to the above defined steps, including MDC, in mM. Use the Wizard tab to calculate concentrations from added volumes.

Description:

This method estimates PN based on known baseline ΔψP in a complete calibration paradigm (K-steps) using the FLIPR Membrane Potential Kit from Molecular Devices (aka PMPI, plasma membrane potential indicator).