Correct Intensity Jump ( IACorrectIntensityJump )
Parameters:
Name |
Short Name |
Type |
Description |
Type |
type |
string |
How to calculate match intensities before and after change. |
Action |
action |
string |
Sets which part of the recording to scale to match the other part. |
Factor |
factor |
real |
If fixed factor is selected in type, this value will be used. |
Background offset |
bgoffset |
real |
|
Frame before jump |
frame |
integer |
The last frame before jump. Frame numbering starts at 1. |
Correct all linked images with the same factor |
correctall |
boolean |
The correction factor is calculated from the input image and all linked images are transformed in the same way. |
Description:
Eliminates a single intensity change (jump) happening between two consecutive frames by multiplying a part (see Action) of the recording with a factor calculated to match the intensity of frames before and after the jump (set by Frame before jump).
The background offset is subtracted before transformation, and then added back to the result.
Matches average intensities of whole frames. Calculates mean intensities of multiple frames, or extrapolates intensities using linear regression to the time point between the last frame before jump and the first frame after the jump.
Mask background for more robust results.