Image processing functions in Image Analyst MKII
Image Analyst MKIIFunctions Glossary
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2D DFT Filter
2D DFT Filter Butterworth BP
2D DFT Filter Butterworth BP Tiled
2D Kernel Convolution
2D Median
2D Morphological Operator
2D Nonlinear Filter
2D Savitzky-Golay filter
Absolute Value
Affine Transformation
Align Channels
Align Series (Image Stabilizer)
Align Tiled Channels
Align Tiled Series (Image Stabilizer)
Anisotropic Diffusion Filter
Attach Intensity Gating Image
Attach Overlay Image
Automatic ROI drawing
Band Pass filter Optimization
Blind Spectral Unmix with NMF
Calculate Simple Crossbleed
Calculate Spatial Moments
Calibration Wizard Parameters
Clear Segmentation Classifiers
Close Image Window
Copy Image Window
Copy ROIs from Other Image
Correct Intensity Jump
Correct Lens Distortion
Count Division and Cell Death
Count Object Colocalization
Create ROI
Create ROIs from Segments
Crop
Crop Image in Place
Crop Image to Segments
Cross-correlation data
Cross-correlation image
Detect Nuclei Convolution
Differential Evolution Optimizer
Distance from Segments
Draw Model Mitochondrion
Draw Random Position Model Mitochondria
EndIf
Erase All ROIs
Excel Window Command
Export
Fill Mask
Fill or Mask Active ROI
FLIPR Calibration with [K+]ec steps and known [K+]ic and known kP
FLIPR Complete Calibration
FLIPR Complete Calibration with known kP
FLIPR Complete Calibration with known kP - Goldman
FLIPR Complete Iterative Calibration
FLIPR Estimate PN
FLIPR Short Calibration based on known potential during MDC and CDC
FLIPR Short Calibration between known baseline and CDC
FLIPR Short Calibration between known baseline and MDC
FLIPR Short Calibration between known baseline and separately measured fP0
FLIPR Short Calibration from Zero with fx=0
FunctionOptions
Get Image Information
Get Linked Channel
If
If Greater Than Zero
Image Arithmetic (image-image math)
Image Arithmetic In Place
Image Arithmetic Single Frame
Inpaint Mask
Input
Invert
Lens Correction Optimization
Link Image Windows
Load and Run Pipeline
Load ROIs
Mask Borders
Mask Frames by Plot Values
Mask Images
Measure Object Intensity
Measure Object Morphology
Mirror or Rotate (new image)
Mirror or Rotate in Place
Multi-Dimensional Open Information
Multi-Dimensional Open Stage Position
Multi-Dimensional Reload Channel
New Image
New Time Scale
Onset Image
Open File
Optical Flow
Options
Pipeline
Pipeline Optimization
Pipeline Optimization Parameter
Plot
Plot Correlation (Colocalization)
Plot Intensities Corresponding to Segments
Plot Morphological Parameters of Segments
Plot Ratio
Plot ROI Dimensions
Plot Tracking Parameters
Potential calibration constants
Potential calibration error propagation
Potential calibration expert overrides
Projection of Vectors from a Point
Ratio
Ratiometric ROI Classifiers
ReCount Division and Cell Death
Reevaluate Segments
Remove Blank Frames
Rename
Resample Image
ROI Classifiers
Run Membrane Potential Calibration
Save ROIs
Scalar Arithmetic (image-value math)
Scalar Arithmetic Multi
Secondary Watershed Segmentation
Select
Select by Number
Sensor Noise Characteristics
Set Reference Image
Set Scaling/LUT
Set Segmentation Classifiers
Set Segmentation Intensity Classifiers
Shift Time Scale
Simple 2D Cross-correlation
Simple Segmentation
Skeletonize
Spectral Unmix
Strip to Well Cell Count
Substitute Poisson Noise
Subtract Background or Normalize
T or Z-project
Template Matching
Temporal Average Filter
Temporal Block Filter
Temporal Median Filter
Temporal Rolling Projection
Temporal Savitzky-Golay Filter
Thinness Ratio Optimization
Threshold
Time Stamp and Scale Bar
TMRM Complete Calibration
TMRM Complete Calibration with known kT
TMRM Complete Calibration with known kT and K-steps
TMRM Short Calibration between known baseline and MDC or CDC
Track Objects
Truncate or Cut
Wait for All Inputs
Watershed Segmentation
Wiener filter
Window Menu Command
Write Back Scaled Values
ΔF/F0

Band Pass filter Optimization ( IAFilterOptimize )

Parameters:
Name Short Name Type Description
Filter type type string The type of the filter to be optimized. Fill in only the relevant cut on and cut off order parameters to the chosen filter type.
Minimum cut on ω lmin real Cut on and cut off ω will be equal or larger than this value.
Maximum cut off ω lmax real Cut on and cut off ω will be equal or smaller than this value.
Cut on order minimum ordonmin real Order of Butterworth filter
Cut on order maximum ordonmax real Order of Butterworth filter
Cut off order minimum ordoffmin real Order of Butterworth filter
Cut off order maximum ordoffmax real Order of Butterworth filter
Spatial freq. (ω) unit units string Sets the units of the above given ω values.
Filter normalization normalization string Normalizes filter coefficients to keep an integral of 1 for the 1D or 2D filter function
Test ROI for attenuation roi1 integer Reads intensities from this ROI for the test
Test ROI for transmission roi2 integer Reads intensities from this ROI for the test
Number of baseline frames baseline integer The image series consists of the given number of baseline frames followed by the given number of treated frames. This can be repeated any time in this order with the same number of frames.
Number of treatment frames treatment integer The image series consists of the given number of baseline frames followed by the given number of treated frames. This can be repeated any time in this order with the same number of frames.
Direction of change in transmission ROI direction string Whether to look for an increasing or a decreasing signal
Weight on signal to noise ratio snrweight real Weight factor for signal to noise ratio component of the optimization (1-z factor between transmitted and attenuated signals)
Preserve edges edges boolean Performs mirrored tiling to prevent edge artifacts. Slows down processing by 4x.
Enlarge paper enlarge boolean Enlarges image by mirrored tiling to quadrangular. Prevents edge artifacts and distortions from non-quadrangular images.
Enlarge to 2^ enlargeto integer Size of image during filtering as power of 2.
Leave only phase phase boolean Sets complex amplitudes in Fourier space to 1 keeping their original arc.
Absolute absolute boolean Calculates absolute value after filtering. Required for intensity measurements on high pass and band pass filtered images.
Protect MASK protectmask boolean Fills up masked areas and undetermined pixel values with zeros before processing. Required for masked images.
Description:
High Pass, Band Pass or Low Pass Filter Optimization.