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FLIPR Short Calibration between known baseline and separately measured fP0
FLIPR Short Calibration from Zero with fx=0
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ΔF/F0

FLIPR Short Calibration between known baseline and separately measured fP0 ( IAFLIPRShortBaselinefP0 )

Parameters:

Name	Short Name	Type	Description
Range definitions in time courses	rangedef	string	Defines whether the ranges given below refer to frames or seconds in the recordings
Baseline Range	BaselineRange	string	Defines the range of the baseline (in the above defined time units). Longer baseline results more accurate calibration.
ΔψP at baseline (mV)	PMP0	real	Previously measured value of ΔψP at baseline.
kP	kP	real	Previously measured value of kp
fP0	fP0	real	Separately measured value of fp0

Description:

This method calculates plasma membrane potential (ΔψP) time course based on previously known baseline potential and kP using the FLIPR Membrane Potential Kit from Molecular Devices (aka PMPI, plasma membrane potential indicator).
Required experimental protocol: baseline, followed by any time lapse. The final complete depolarization is measured in a separate sample starting from an identical baseline.
This calibration is recommended only for averaged fluorescence of many cells and not for individual cells, because individual cell fP0 value is variable.