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FLIPR Short Calibration between known baseline and CDC
FLIPR Short Calibration between known baseline and MDC
FLIPR Short Calibration between known baseline and separately measured fP0
FLIPR Short Calibration from Zero with fx=0
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ΔF/F0

FLIPR Short Calibration between known baseline and MDC ( IAFLIPRShortBaselineMDC )

Parameters:

Name	Short Name	Type	Description
Range definitions in time courses	rangedef	string	Defines whether the ranges given below refer to frames or seconds in the recordings
Baseline Range	BaselineRange	string	Defines the range of the baseline (in the above defined time units). Longer baseline results more accurate calibration.
Keq Range	KeqRange	string	The range where K+ equilibrium potential is established (in the above defined time units).
ΔψP at baseline (mV)	PMP0	real	Previously measured value of ΔψP at baseline.
ΔψP at Keq (mV)	PMP1	real	Previously measured value of ΔψP during K+ equilibrium or MDC treatment.
kP	kP	real	Previously measured value of kp
fP0-fPX Allowed Range	fP0MinusfPXRange	string	Trace selection criterion: the minimum and maximum allowed value of fP0-fPX

Description:

This method calculates plasma membrane potential (ΔψP) time course based on previously determined baseline potential, know potential during established K-equilibrium potential or MDC treatment and kP using the FLIPR Membrane Potential Kit from Molecular Devices (aka PMPI, plasma membrane potential indicator). This method does not require measurement of FLIPR fluorescence during complete depolarization.
Required experimental protocol: baseline, followed by any time lapse, followed by establishment of known K-equilibrium potential.