Name |
Short Name |
Type |
Description |
Condition label override |
ConditionLabel |
string |
Condition label to override the entry from the Multi-Dimensional Open dialog, also shown in the Membrane Potential Calibration Wizards caption. |
Uncoupler label |
UNC |
string |
Label string, only letter characters, as show in the condition name, followed by digits of the concentration in µM, serial additions separated with ";". Not case sensitive. |
ATP-synthase inhibitor label |
OLI |
string |
Label string, only letter characters, as show in the condition name, followed by digits of the concentration, serial additions separated with ";". Not case sensitive. |
Glycolysis inhibitor label |
IODO |
string |
Label string, only letter characters, as show in the condition name, followed by digits of the concentration, serial additions separated with ";". Not case sensitive. |
Minimal ΔψM |
MMPMin |
real |
More depolarized potentials will be excluded from the analysis. |
Ub override |
UB |
real |
Override to set the ΔψM measured in the presence of oligomycin. Provide a value in negative millivolts. 0 turns off feature. This value will be used if no "ATP-synthase inhibitor label" is found in condition labels. |
Force fit through baseline point |
forcefitbaseline |
boolean |
Forces the baseline datapoint to be the y-axis intercept (1/ΔψM). If No, Um or Ub (depending on the paradigm) will be replaced with 1/intercept value. When extrapolation is used below, this option is not applied to the data. |
Extrapolate Ub to estimate leak |
extrapolate |
boolean |
Calculates first data point from data in the presence of uncoupler; Um or Ub in single well mode. Set this Yes if having only oligomycin+uncoupler points, or the oligomycin point is erroneously measured. Or if Um is liely burdened by increased proton leak... |