TMRM Complete Calibration with known kT and K-steps ( IATMRMCompleteKTKSteps )

Parameters:

Name	Short Name	Type	Description
Range definitions in time courses	rangedef	string	Defines whether the ranges given below refer to frames or seconds in the recordings
Baseline Range	BaselineRange	string	Defines the range of the baseline (in the above defined time units). Longer baseline results more accurate calibration.
fT0-fTX Allowed Range	fT0MinusfTXRange	string	Trace selection criterion: the minimum and maximum allowed value of fT0-fTX
VF (mitochondria:cell volume fraction)	VF	real	Confocal stereologic volume fraction of mitochondria (inner boundary membrane) to whole cell (including nucleus)
VFM (matrix:mitochondrion volume fraction)	VFM	real	EM stereologic volume fraction of matrix to mitochondrion (inner boundary membrane)
aR` (matrix:cytosol apparent activity coefficient ratio)	aR	real	Apparent activity coefficient ratio measured by confocal microscopy
Quality control based on MDC calibrates to zero	QCMDC	boolean	Removes traces where MDC does not calibrate to zero, or noisy. Set threshold below.
Maximum deviation from zero (mV)	QCMDCdeviation	real	Traces where the absolute mean or SD of MDC Range is larger than this number are removed.
kT	kT	real	Previously measured value of kT
TMRM r^2 Min	TMRMr2Min	real	Trace selection criterion: the minimum allowed value of r^2 for the TMRM-regression.
Quality control by propagated error of baseline	QCMMPrest	boolean	Removes traces where the estimated SE of baseline is greater than the threshold below. Requires error propagation.
Maximum SE of baseline (mV)	QCMMPrestMaxSE	real	Traces where the SE of resting ΔψM parameter is larger than this number are removed.

Description:

This method calculates mitochondrial membrane potential (ΔψM) time course by calibration parameters from a single time lapse with the exception of kT.
Required experimental protocol: any time lapse, followed by mitochondrial depolarization, but no requirement for well resolved decay curve of TMRM fluorescence. Requires final complete depolarization.
Determination of resting mitochondrial membrane potential requires baseline recording. Longer baseline results more accurate determination. If the baseline is not steady, use the "Do not assume equilibrium baseline" switch in the Constants tab.
Cells have to be able to maintain some ΔψP during MDC addition. Very depolarized ΔψP leads to inaccurate or impossible calibration.