Image processing pipelines in Image Analyst MKII
Image Analyst MKIIPipelines Glossary
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Measure intensity of membranes

Parameters:
Name # Type Description
Sensitivity factor 1 (gamma) 1 real Gamma value for scaling images before binarization typically 1-2.
Sensitivity factor 2 (Otsu factor) 2 real Factor for threshold determination, smaller values result increased sensitivity to find membranes.
Thickness (pixels) 3 integer Diameter of the structuring element in pixels
Description:
Masks image with a skeletonized copy of the original image.
Plots the mean intensity of the masked image.