Track segments with one intensity channel

Parameters:

Name	#	Type	Description
Channel Number of segmented image to track	1	integer	The first linked image window with matching channel number will be invoked.
Channel Number of intensities (helps matching objects)	2	integer	The first linked image window with matching channel number will be invoked.
Maximal distance that a cell travels between consecutive frames (pixels)	3	real	Largest distance in pixels that objects may move between frames
Minimum track length (frames)	4	integer	Removes segments that are only present in the given number of frames

Description:

This pipeline tracks already segmented objects (cells or nuclei) a time lapse. There is no requirement for frame-to-frame overlap between objects, therefore image sequences recorded by longer acquisition intervals can be also used. To help distinguishing objects as they move frame to frame, an additional intensity channel is taken into account.
Hint: Use this pipeline with the subsequent application of the “Plot Intensities Corresponding to Segments” to measure fluorescence intensity time courses in moving cells.

Keywords: cell motility, cell movement, cell migration, cellular motility, cellular movement, cellular migration