Video or image export 1-4 channels with local background subtraction with fixed scaling

Parameters:

Name	#	Type	Description
Channel #1 Color (Look Up Table)	1	string	Look Up Table: "Grayscale", "Red", "Green", "Blue", "Pseudocolor", "Intensity Gated Pseudocolor", "Fire", "ROI color", "Off"
Channel #2 Color (Look Up Table)	2	string	Look Up Table: "Grayscale", "Red", "Green", "Blue", "Pseudocolor", "Intensity Gated Pseudocolor", "Fire", "ROI color", "Off"
Channel #3 Color (Look Up Table)	3	string	Look Up Table: "Grayscale", "Red", "Green", "Blue", "Pseudocolor", "Intensity Gated Pseudocolor", "Fire", "ROI color", "Off"
Channel #4 Color (Look Up Table)	4	string	Look Up Table: "Grayscale", "Red", "Green", "Blue", "Pseudocolor", "Intensity Gated Pseudocolor", "Fire", "ROI color", "Off"
Show only composite image	5	boolean	Yes executes output #1, No executes output #2.
Subtract reference image background	6	boolean	Yes executes output #1, No executes output #2.
Local background subtraction method	7	string	The Nth output will be executed when the Nth value is selected here from a ;-separated list given below at "Possible selector values".
Rolling ball: median filter size	8	integer	The median is calculated over a width x width rectangular area around each pixel of the image.
Spatial filtering: Number of tiles in x	9	integer	The image consists of this number of equal sized tiles in x dimension.
Spatial filtering: Number of tiles in y	10	integer	The image consists of this number of equal sized tiles in y dimension.
Spatial filtering: Largest object size (for background removal, pixels times number of tiles)	11	real	Size of the object to be passed by the filtering. The pixel size must be multiplied by the number if tiles (in x or y). Objects larger than this will be removed as background. Cut on of the band pass Butterworth filter.
Global background level (Percentile)	12	real	Background is calculated as frame-by-frame mean of those pixels that are the darker than this percentile of image histogram for the duration of the entire recording.
Channel #1 Scaling Max value	13	real	Pixel intensity for the maximum of the Look Up Table.
Channel #2 Scaling Max value	14	real	Pixel intensity for the maximum of the Look Up Table.
Channel #3 Scaling Max value	15	real	Pixel intensity for the maximum of the Look Up Table.
Channel #4 Scaling Max value	16	real	Pixel intensity for the maximum of the Look Up Table.
Show time stamp	17	boolean	Turns on time stamps (set font in the Preferences Dialog/Misc tab).
Time stamp format	18	string	Time will appear like this: "0 s", "0.0 s", "0.00 s", "0.000 s", "0000 ms", "00'00"", "00'00"00", "00h00min"
Show scale bar	19	boolean	Turns on scale bar.
Scale bar unit	20	string	Available units: "nm", "µm", "mm", "cm", "m".
Scale bar size	21	real	Length of the scale bar in the units given above.
Export image/video	22	boolean	Yes executes output #1, No executes output #2.
Export Filename	23	string
Video or Image Format	24	string	Available formats: "BMPPadded", "BMP", "AVI", "JPG", "JPGPadded"

Description:

This pipeline removes inhomogeneity of background with high pass filtering, scales the image with the Look Up Table (LUT) of choice, applies time stamp, scale bar and exports in the format of choice. The tiled high pass filter can efficiently remove pattern originating from tiled imaging using wide field microscopy.
Set time stamp font in the Preferences Dialog/Misc tab.
Set video or jpg compression in the Preferences Dialog/Export tab. Note: uncheck ‘Show compression dialog’ for automated processing of multiple positions.
Note: if using x264vfw, please check Zero Latency in its configuration panel.
Note: some video formats require image width divisible by 4 and height divisible by 2.
Note: See help on expression evaluation to set up automatic file naming.

This pipeline was chnaged comparted to the parent "Video or image export 1-4 channels with local background subtraction" by changing image scaling from automatic (Percentile of the entire image series) to fixed scaling. Background is removed (=0) and the scale maximum can be adjusted for each channel.