Video or image export 1-4 channels with local background subtraction cropped, stabilized

Parameters:

Name	#	Type	Description
Channel #1 Color (Look Up Table)	1	string	Look Up Table: "Grayscale", "Red", "Green", "Blue", "Pseudocolor", "Intensity Gated Pseudocolor", "Fire", "ROI color", "Off"
Channel #2 Color (Look Up Table)	2	string	Look Up Table: "Grayscale", "Red", "Green", "Blue", "Pseudocolor", "Intensity Gated Pseudocolor", "Fire", "ROI color", "Off"
Channel #3 Color (Look Up Table)	3	string	Look Up Table: "Grayscale", "Red", "Green", "Blue", "Pseudocolor", "Intensity Gated Pseudocolor", "Fire", "ROI color", "Off"
Channel #4 Color (Look Up Table)	4	string	Look Up Table: "Grayscale", "Red", "Green", "Blue", "Pseudocolor", "Intensity Gated Pseudocolor", "Fire", "ROI color", "Off"
Crop image	5	boolean	Yes crops images according to the parameters below
Crop: Left (pixels)	6	real	Distance in pixels
Crop: Top (pixels)	7	real	Distance in pixels
Crop: Width (pixels)	8	real	Distance in pixels
Crop: Height (pixels)	9	real	Distance in pixels
Align (stabilize) image series	10	boolean	Yes performs image series alignment according to the parameters below
Align image series based on channel number	11	integer	The first linked image window with matching channel number will be invoked.
Alignment: Compared to first frame	12	boolean	Dislocations are calculated as compared to the first frame.
Alignment: Difference Period (of not to first frame)	13	integer	Difference period gives frames how far from each other are compared. N/A if alignment if based on comparison to the first frame.
Show only composite image	14	boolean	Yes executes output #1, No executes output #2.
Subtract reference image background	15	boolean	Yes executes output #1, No executes output #2.
Local background subtraction method	16	string	The Nth output will be executed when the Nth value is selected here from a ;-separated list given below at "Possible selector values".
Rolling ball: median filter size	17	integer	The median is calculated over a width x width rectangular area around each pixel of the image.
Spatial filtering: Number of tiles in x	18	integer	The image consists of this number of equal sized tiles in x dimension.
Spatial filtering: Number of tiles in y	19	integer	The image consists of this number of equal sized tiles in y dimension.
Spatial filtering: Largest object size (for background removal, pixels times number of tiles)	20	real	Size of the object to be passed by the filtering. The pixel size must be multiplied by the number if tiles (in x or y). Objects larger than this will be removed as background. Cut on of the band pass Butterworth filter.
Global background level (Percentile)	21	real	Background is calculated as frame-by-frame mean of those pixels that are the darker than this percentile of image histogram for the duration of the entire recording.
Scaling, Min percentile	22	real	This percentile of the image histogram sets the intensity value where the minimum of the Look Up Table (LUT) is scaled. Use -1 to override this with fixed value set below at "Min value".
Scaling, Max percentile	23	real	This percentile of the image histogram sets the intensity value where the maximum of the Look Up Table (LUT) is scaled. Use -1 to override this with fixed value set below at "Max value".
Show time stamp	24	boolean	Turns on time stamps (set font in the Preferences Dialog/Misc tab).
Time stamp format	25	string	Time will appear like this: "0 s", "0.0 s", "0.00 s", "0.000 s", "0000 ms", "00'00"", "00'00"00", "00h00min"
Show scale bar	26	boolean	Turns on scale bar.
Scale bar unit	27	string	Available units: "nm", "µm", "mm", "cm", "m".
Scale bar size	28	real	Length of the scale bar in the units given above.
Export image/video	29	boolean	Yes executes output #1, No executes output #2.
Export Filename	30	string
Video or Image Format	31	string	Available formats: "BMPPadded", "BMP", "AVI", "JPG", "JPGPadded"

Description:

This pipeline removes inhomogeneity of background with high pass filtering, scales the image with the Look Up Table (LUT) of choice, applies time stamp, scale bar and exports in the format of choice. The tiled high pass filter can efficiently remove pattern originating from tiled imaging using wide field microscopy.
Set time stamp font in the Preferences Dialog/Misc tab.
Tiled images: the background tiling pattern is efficiently removed by spatial filtering if the recording was performed without overlap and image registration. Provide the number of tiles in x and y direction.
Note: See help on expression evaluation to set up automatic file naming.

V2:
Changed Export filename