Image processing pipelines in Image Analyst MKII
Image Analyst MKIIPipelines Glossary
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Subtract background of TILED images using 2DDFT non-calibrated.ips

Parameters:
Name # Type Description
Number of tiles in x 1 integer The image consists of this number of equal sized tiles in x dimension.
Number of tiles in y 2 integer The image consists of this number of equal sized tiles in y dimension.
Largest object size (pixels times number of tiles) 3 real Size of the object to be passed by the filtering. The pixel size must be multiplied by the number if tiles (in x or y). Cut on of the band pass Butterworth filter.
Calculate absolute values 4 boolean Calculates absolute value after filtering. Required for intensity measurements on high pass and band pass filtered images.
Description:
Removes patterned background originating from tiling using high pass filtering.
Uses pixels size information to generate filter for background suppression. Optimal for tiled images that are composed of quadrangular frames sized power of 2.
Due to a current limitation in the pipeline parametering, the object size must be entered as the real object size in pixels multiplied by the number if tiles (in x or in y).