Image processing pipelines in Image Analyst MKII
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Subtract background of TILED images using 2DDFT calibrated.ips

Parameters:
Name # Type Description
Number of tiles in x 1 integer The image consists of this number of equal sized tiles in x dimension.
Number of tiles in y 2 integer The image consists of this number of equal sized tiles in y dimension.
Typical object size (microns) 3 real Cut on of the band pass Butterworth filter
Calculate absolute values 4 boolean Calculates absolute value after filtering. Required for intensity measurements on high pass and band pass filtered images.
Description:
Removes patterned background originating from tiling using high pass filtering.
Uses pixels size information to generate filter for background suppression. Optimal for tiled images that are composed of quadrangular frames sized power of 2.