Image processing pipelines in Image Analyst MKII
Image Analyst MKIIPipelines Glossary
HomeWorkflowImage Processing BasicsFunctions GlossaryPipelines GlossaryProtocolsQuick How ToSearch

Optical density of tissue section - create reference image for OD BLANK using median

Parameters:
Name # Type Description
Method: 1 string Choose median or minimum.
Description:
This pipeline generates background reference image from multi position recordings on low density cultures. Because cells positioned randomly, calculation of median (or minimum for more dense cultures) results the background intensities.
Open one channel at a time in the Multi-Dimensional Open dialog to calculate image background. Repeat this for all channels. Use this only if cell cultures are subconfluent.