Image processing pipelines in Image Analyst MKII
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MitoSOX MitoTracker Deep Red Ratio End Point POS. CTRL. REFERENCE

Parameters:
Name # Type Description
Channel Number for MitoSOX 1 integer The first linked image window with matching channel number will be invoked.
Channel Number for MitoTracker Deep Red 2 integer The first linked image window with matching channel number will be invoked.
Channel Number for Hoechst 3 integer The first linked image window with matching channel number will be invoked.
Background Level (percentile) 4 real Background is calculated as frame-by-frame subtraction of the given percentile of the image intensity histogram.
Description:
This pipeline sets up of positive control (high ROS, high cell death).
Using Histogram Reference Images ensures even segmentation and elimination of debris and dead cells in all wells (stage positions) when using the main pipeline in a microplate setting, and cell density or amounts of dead cells may vary well to well. Please use images with considerable amount of debris and dead cells to generate reference images, or maximum intensity project a set of stage positions (using the Editing T or Z Project function). Please use the same background level for reference image generation as in the main pipeline.