Local background subtraction with median filtering (rolling ball)
Parameters:
| Name |
# |
Type |
Description |
| Median filter size (pixels) |
1 |
integer |
A copy of the original image is median filtered with this window size, and subtracted from the original image. |
Description:
Subtracts local background in each frame. The median filter size has to be substantially larger than the size of the cells or details where fluorescence will be measured after background subtraction. Use this background subtraction method to suppress fluorescence from inhomogeneous background, such a Geltrex coated dishes, or floating debris. Similar to rolling ball background subtraction.
Pipelines Glossary
