Fraction of cell death time lapse POS. CTRL. REFERENCE

Parameters:

Name	#	Type	Description
Channel of cell death probe	1	integer	The first linked image window with matching channel number will be invoked.
Background Level, (percentile)	2	real	Background is calculated as percentile for each frame.
Use inhomogeneous background removal with spatial filtering	3	boolean	Yes executes output #1, No executes output #2.
Spatial filtering: Number of tiles in x	4	integer	The image consists of this number of equal sized tiles in x dimension.
Spatial filtering: Number of tiles in y	5	integer	The image consists of this number of equal sized tiles in y dimension.
Spatial filtering: Largest object size (pixels times number of tiles)	6	real	Cut on of the band pass Butterworth filter

Description:

Reference image calculation for the corresponding pipeline.
The reference image is a positive control, with a strong fluorescence signal. This pipeline results in Histogram Reference Images that will be used for uniform scaling of all images in the assay. Using Histogram Reference Images reduces the well-to-well variability of the readout. It can be also the same image that is to be processed with the corresponding pipeline.