Image processing pipelines in Image Analyst MKII
Image Analyst MKIIPipelines Glossary
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Fraction of cell death time lapse POS. CTRL. REFERENCE

Parameters:
Name # Type Description
Channel of cell death probe 1 integer The first linked image window with matching channel number will be invoked.
Background Level, (percentile) 2 real Background is calculated as percentile for each frame.
Use inhomogeneous background removal with spatial filtering 3 boolean Yes executes output #1, No executes output #2.
Spatial filtering: Number of tiles in x 4 integer The image consists of this number of equal sized tiles in x dimension.
Spatial filtering: Number of tiles in y 5 integer The image consists of this number of equal sized tiles in y dimension.
Spatial filtering: Largest object size (pixels times number of tiles) 6 real Cut on of the band pass Butterworth filter
Description:
Reference image calculation for the corresponding pipeline.
The reference image is a positive control, with a strong fluorescence signal. This pipeline results in Histogram Reference Images that will be used for uniform scaling of all images in the assay. Using Histogram Reference Images reduces the well-to-well variability of the readout. It can be also the same image that is to be processed with the corresponding pipeline.