Image processing pipelines in Image Analyst MKII
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Create reference image for OD BLANK and FLUORESCENCE BG using median

Parameters:
Name # Type Description
Oil-Red-O fluorescence channel number 1 integer The first linked image window with matching channel number will be invoked.
Hematoxylin brightfield channel number 2 integer The first linked image window with matching channel number will be invoked.
Method: 3 string Choose median or minimum.
Description:
This pipeline generates background reference image from multi position recordings on low density cultures. Because cells positioned randomly, calculation of median (or minimum for more dense cultures) results the background intensities.
Open one channel at a time in the Multi-Dimensional Open dialog to calculate image background. Repeat this for all channels. Use this only if cell cultures are subconfluent.
This pipeline is used by "Oil-red-O Fat droplet analysis".