Count live, apoptotic or necrotic cells POS. CTRL. REFERENCE
Parameters:
| Name |
# |
Type |
Description |
| Channel of apoptosis probe |
1 |
integer |
The first linked image window with matching channel number will be invoked. |
| Channel of necrosis probe |
2 |
integer |
The first linked image window with matching channel number will be invoked. |
| Number of tiles in x |
3 |
integer |
The image consists of this number of equal sized tiles in x dimension. |
| Number of tiles in y |
4 |
integer |
The image consists of this number of equal sized tiles in y dimension. |
| Background Level, (percentile) |
5 |
real |
|
Description:
Positive Control reference image calculation for the correspondingly named pipeline.
The reference image is a positive control, with a strong fluorescence signal. This pipeline results in Histogram Reference Images that will be used for uniformly scaling all images in the assay. Using Histogram Reference Images reduces the well-to-well variability of the readout. It can be also the same image that is to be processed with the corresponding pipeline.
This pipeline also uses Background Reference Images that must be generated before running this pipeline. Please generate background reference image by setting it in an Image Window by right-click/Set as Reference Image/Background, or use the “Create reference image for multiwell plate using median” pipeline to automatically generate background from multi position image sets.
Tiled images: the background tiling pattern is efficiently removed by spatial filtering if the recording was performed without overlap and image registration. Provide the number of tiles in x and y direction.
Version History:
V2: updated description
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