Image processing pipelines in Image Analyst MKII
Image Analyst MKIIPipelines Glossary
HomeWorkflowImage Processing BasicsFunctions GlossaryPipelines GlossaryProtocolsQuick How ToSearch

Brightfield processing Hematoxylin+DAB

Parameters:
Name # Type Description
Coefficient Matrix 1 string The matrix can be defined as Convolution kernels in the Preferences dialog, or define it here as ={{1,"probe 2 to channel 1",..},{"probe 1 to channel 2",1,..},....}
Description:
This snippet converts an RGB (3 channel) bright filed image into 2 fluorescence-like channels by unmixing two major components, e.g. hematoxylin and DAB staining. The pipeline uses only the red and blue channels for this. Use the two “Get Linked Channel” functions at the bottom to add more processing. The resultant inverted and unmixed images are ready for segmentation.